Record details

Title
    Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres for electrochemical detection of proteins
Author
    Bílková, Z.
    Čadková, M.
    Horák, Daniel
    Korecká, L.
    Kovářová, Jana
    Metelka, R.
    Petrovský, Eduard
    Šálek, Petr
Language
    anglicky
Publication type
    článek v odborném periodiku
Source title - serial
    Journal of Materials Chemistry
Vol./nr.
    Roč. 21, č. 38
Pages
    s. 14783-14792
Year
    2011
Notes
    Projekt: GA203/09/0857, GA ČR3cav_un_auth*0251121
    Projekt: GA203/09/1242, GA ČR3cav_un_auth*0250336
    Rozsah: 10 s.
    Výzkumný záměr: CEZ:AV0Z30120515
    Výzkumný záměr: CEZ:AV0Z40500505
Subject category
    divinylbenzene
    microspheres
    styrene
Keyword
    Detection
    Electrochemical
    Hypercrosslinked
    Immunomagnetic
    Microspheres
    Polystyrene
    Proteins
    Sulfonated
Abstract (in english)
   Poly(styrene-co-divinylbenzene) microspheres of narrow size distribution were prepared by (2-hydroxypropyl)cellulose-stabilized dispersion copolymerization of styrene and divinylbenzene in a 2-methoxyethanol/ethanol mixture under continuous addition of divinylbenzene. The copolymerization was initiated with dibenzoyl peroxide. The obtained microspheres were chloromethylated and then hypercrosslinked. Their porous structure was analyzed by nitrogen adsorption and mercury porosimetry. Superparamagnetic iron oxide nanoparticles were precipitated within the pores of microspheres from Fe(II) and Fe(III) chloride solution. The Fe content in the microspheres was determined by carbon analysis, atomic absorption spectroscopy, thermogravimetric analysis. Magnetic properties of the microspheres were characterized. Sulfo groups were introduced into the microspheres to prepare an immunomagnetic electrochemical biosensor for protein detection with ovalbumin as a model substance.
Contributor
    AV ČR Brno, Geofyzikální ústav
Contributor code
    AV ČR, GFÚ
Source format
    U
Import date
    9. 12. 2013