Record details

Title
    Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria
Author
    Jehlička, Jan
    Oren, Aharon
    Vítek, Petr
Language
    anglicky
Publication type
    článek v periodiku
Source title - serial
    Extremophiles : life under extreme conditions
Vol./nr.
    Roč. 16, č. 3
Pages
    8
Year
    2012
Thesaurus term
    Compatible solutes
    ectoine
    Ectothiorhodospira
    glycine betaine
    Halomonas
    Raman spectroscopy
Keyword
    Bacteria
    Compatible
    Halophilic
    Identification
    Raman
    Solutes
    Spectroscopy
    Use
Abstract (in english)
   We explored the use of Raman spectroscopy to detect organic osmotic solutes as biomarkers in the moderately halophilic heterotrophic bacterium grown in complex medium (accumulation of glycine betaine) and in defined medium with glucose as carbon source (biosynthesis of ectoine), and in the anoxygenic phototrophic known to synthesize glycine betaine in combination with minor amounts of trehalose and -alpha-carbamoyl glutamineamide. We tested different methods of preparation of the material: lyophilization, two-phase extraction of water-soluble molecules, and perchlorate extraction. Raman signals of glycine betaine and ectoine were detected; perchlorate extraction followed by desalting the extract on an ion retardation column gave the best results. Lyophilized cells of showed strong signals of carotenoid pigments, and glycine betaine could be detected only after perchlorate extraction and desalting. The data presented show that Raman spectroscopy is a suitable tool to assess the mode of
   osmotic adaptation used by halophilic microorganisms.
Contributor
    UK Praha, Přírodovědecká fakulta
Contributor code
    UK, PřF
Source format
    R
Import date
    27. 4. 2014