Record details

    Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria
    Jehlička, Jan
    Oren, Aharon
    Vítek, Petr
Publication type
    článek v periodiku
Source title - serial
    Extremophiles : life under extreme conditions
    Roč. 16, č. 3
Thesaurus term
    Compatible solutes
    glycine betaine
    Raman spectroscopy
Abstract (in english)
   We explored the use of Raman spectroscopy to detect organic osmotic solutes as biomarkers in the moderately halophilic heterotrophic bacterium grown in complex medium (accumulation of glycine betaine) and in defined medium with glucose as carbon source (biosynthesis of ectoine), and in the anoxygenic phototrophic known to synthesize glycine betaine in combination with minor amounts of trehalose and -alpha-carbamoyl glutamineamide. We tested different methods of preparation of the material: lyophilization, two-phase extraction of water-soluble molecules, and perchlorate extraction. Raman signals of glycine betaine and ectoine were detected; perchlorate extraction followed by desalting the extract on an ion retardation column gave the best results. Lyophilized cells of showed strong signals of carotenoid pigments, and glycine betaine could be detected only after perchlorate extraction and desalting. The data presented show that Raman spectroscopy is a suitable tool to assess the mode of
   osmotic adaptation used by halophilic microorganisms.
    UK Praha, Přírodovědecká fakulta
Contributor code
    UK, PřF
Source format
Import date
    27. 4. 2014